Coding

Part:BBa_K4780000

Designed by: Jack Dalton   Group: iGEM23_ANU-Australia   (2023-09-26)


This is a synthetic arsenic binding protein based designed using the protein MPNN neural network.

This is a synthetic arsenic binding protein designed using the protein MPNN neural network. Did not express well in E. coli DE3 cells. We switched to T7 Shuffle cells as they express cysteine rich proteins better and this resulted in strong expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 84
    Illegal NgoMIV site found at 176
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None